RapamycinFungusV1, Main, Exploration, bibRecord, 000346

Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.

Identifieur interne : 000346 ( Main/Exploration ); précédent : 000345; suivant : 000347

Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.

Auteurs : Yusuf Sürmeli [Turquie] ; Can Holyavkin [Turquie] ; Alican Topalo Lu [Turquie] ; Mevlüt Arslan [Turquie] ; Halil Brahim K Sakesen [Turquie] ; Zeynep Petek Çakar [Turquie]

Source :

World journal of microbiology & biotechnology [ 1573-0972 ] ; 2019.

RBID : pubmed:31728740

Descripteurs français

KwdFr :
- Acroléine (analogues et dérivés), Acroléine (pharmacologie), Antimycine A (analogues et dérivés), Antimycine A (pharmacologie), Caféine (pharmacologie), Cycloheximide (pharmacologie), Facteurs de transcription (génétique), Ingénierie des protéines (méthodes), Mutagenèse (MeSH), Polymorphisme de nucléotide simple (MeSH), Protéines de Saccharomyces cerevisiae (génétique), Protéines de liaison à l'ADN (génétique), Protéines du cycle cellulaire (génétique), Résistance des champignons aux médicaments (génétique), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Saccharomyces cerevisiae (génétique), Sirolimus (pharmacologie), Stress physiologique (MeSH), Séquençage du génome entier (MeSH), Transcriptome (MeSH), Transporteurs ABC (génétique).
MESH :
- analogues et dérivés : Acroléine, Antimycine A.
- effets des médicaments et des substances chimiques : Saccharomyces cerevisiae.
- génétique : Facteurs de transcription, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ADN, Protéines du cycle cellulaire, Résistance des champignons aux médicaments, Saccharomyces cerevisiae, Transporteurs ABC.
- méthodes : Ingénierie des protéines.
- pharmacologie : Acroléine, Antimycine A, Caféine, Cycloheximide, Sirolimus.
- Mutagenèse, Polymorphisme de nucléotide simple, Stress physiologique, Séquençage du génome entier, Transcriptome.

English descriptors

KwdEn :
- ATP-Binding Cassette Transporters (genetics), Acrolein (analogs & derivatives), Acrolein (pharmacology), Antimycin A (analogs & derivatives), Antimycin A (pharmacology), Caffeine (pharmacology), Cell Cycle Proteins (genetics), Cycloheximide (pharmacology), DNA-Binding Proteins (genetics), Drug Resistance, Fungal (genetics), Mutagenesis (MeSH), Polymorphism, Single Nucleotide (MeSH), Protein Engineering (methods), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae Proteins (genetics), Sirolimus (pharmacology), Stress, Physiological (MeSH), Transcription Factors (genetics), Transcriptome (MeSH), Whole Genome Sequencing (MeSH).
MESH :
- chemical , analogs & derivatives : Acrolein, Antimycin A.
- chemical , genetics : ATP-Binding Cassette Transporters, Cell Cycle Proteins, DNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Transcription Factors.
- chemical , pharmacology : Acrolein, Antimycin A, Caffeine, Cycloheximide, Sirolimus.
- drug effects : Saccharomyces cerevisiae.
- genetics : Drug Resistance, Fungal, Saccharomyces cerevisiae.
- methods : Protein Engineering.
- Mutagenesis, Polymorphism, Single Nucleotide, Stress, Physiological, Transcriptome, Whole Genome Sequencing.

Abstract

Caffeine is a naturally occurring alkaloid, where its major consumption occurs with beverages such as coffee, soft drinks and tea. Despite a variety of reports on the effects of caffeine on diverse organisms including yeast, the complex molecular basis of caffeine resistance and response has yet to be understood. In this study, a caffeine-hyperresistant and genetically stable Saccharomyces cerevisiae mutant was obtained for the first time by evolutionary engineering, using batch selection in the presence of gradually increased caffeine stress levels and without any mutagenesis of the initial population prior to selection. The selected mutant could resist up to 50 mM caffeine, a level, to our knowledge, that has not been reported for S. cerevisiae so far. The mutant was also resistant to the cell wall-damaging agent lyticase, and it showed cross-resistance against various compounds such as rapamycin, antimycin, coniferyl aldehyde and cycloheximide. Comparative transcriptomic analysis results revealed that the genes involved in the energy conservation and production pathways, and pleiotropic drug resistance were overexpressed. Whole genome re-sequencing identified single nucleotide polymorphisms in only three genes of the caffeine-hyperresistant mutant; PDR1, PDR5 and RIM8, which may play a potential role in caffeine-hyperresistance.

DOI: 10.1007/s11274-019-2762-2
PubMed: 31728740

Affiliations:

Turquie

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP-Binding Cassette Transporters (genetics)</term>
<term>Acrolein (analogs & derivatives)</term>
<term>Acrolein (pharmacology)</term>
<term>Antimycin A (analogs & derivatives)</term>
<term>Antimycin A (pharmacology)</term>
<term>Caffeine (pharmacology)</term>
<term>Cell Cycle Proteins (genetics)</term>
<term>Cycloheximide (pharmacology)</term>
<term>DNA-Binding Proteins (genetics)</term>
<term>Drug Resistance, Fungal (genetics)</term>
<term>Mutagenesis (MeSH)</term>
<term>Polymorphism, Single Nucleotide (MeSH)</term>
<term>Protein Engineering (methods)</term>
<term>Saccharomyces cerevisiae (drug effects)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Sirolimus (pharmacology)</term>
<term>Stress, Physiological (MeSH)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcriptome (MeSH)</term>
<term>Whole Genome Sequencing (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acroléine (analogues et dérivés)</term>
<term>Acroléine (pharmacologie)</term>
<term>Antimycine A (analogues et dérivés)</term>
<term>Antimycine A (pharmacologie)</term>
<term>Caféine (pharmacologie)</term>
<term>Cycloheximide (pharmacologie)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Ingénierie des protéines (méthodes)</term>
<term>Mutagenèse (MeSH)</term>
<term>Polymorphisme de nucléotide simple (MeSH)</term>
<term>Protéines de Saccharomyces cerevisiae (génétique)</term>
<term>Protéines de liaison à l'ADN (génétique)</term>
<term>Protéines du cycle cellulaire (génétique)</term>
<term>Résistance des champignons aux médicaments (génétique)</term>
<term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Sirolimus (pharmacologie)</term>
<term>Stress physiologique (MeSH)</term>
<term>Séquençage du génome entier (MeSH)</term>
<term>Transcriptome (MeSH)</term>
<term>Transporteurs ABC (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Acrolein</term>
<term>Antimycin A</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>ATP-Binding Cassette Transporters</term>
<term>Cell Cycle Proteins</term>
<term>DNA-Binding Proteins</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acrolein</term>
<term>Antimycin A</term>
<term>Caffeine</term>
<term>Cycloheximide</term>
<term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acroléine</term>
<term>Antimycine A</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance, Fungal</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ADN</term>
<term>Protéines du cycle cellulaire</term>
<term>Résistance des champignons aux médicaments</term>
<term>Saccharomyces cerevisiae</term>
<term>Transporteurs ABC</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Ingénierie des protéines</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acroléine</term>
<term>Antimycine A</term>
<term>Caféine</term>
<term>Cycloheximide</term>
<term>Sirolimus</term>
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<term>Polymorphism, Single Nucleotide</term>
<term>Stress, Physiological</term>
<term>Transcriptome</term>
<term>Whole Genome Sequencing</term>
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<term>Polymorphisme de nucléotide simple</term>
<term>Stress physiologique</term>
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<front><div type="abstract" xml:lang="en">Caffeine is a naturally occurring alkaloid, where its major consumption occurs with beverages such as coffee, soft drinks and tea. Despite a variety of reports on the effects of caffeine on diverse organisms including yeast, the complex molecular basis of caffeine resistance and response has yet to be understood. In this study, a caffeine-hyperresistant and genetically stable Saccharomyces cerevisiae mutant was obtained for the first time by evolutionary engineering, using batch selection in the presence of gradually increased caffeine stress levels and without any mutagenesis of the initial population prior to selection. The selected mutant could resist up to 50 mM caffeine, a level, to our knowledge, that has not been reported for S. cerevisiae so far. The mutant was also resistant to the cell wall-damaging agent lyticase, and it showed cross-resistance against various compounds such as rapamycin, antimycin, coniferyl aldehyde and cycloheximide. Comparative transcriptomic analysis results revealed that the genes involved in the energy conservation and production pathways, and pleiotropic drug resistance were overexpressed. Whole genome re-sequencing identified single nucleotide polymorphisms in only three genes of the caffeine-hyperresistant mutant; PDR1, PDR5 and RIM8, which may play a potential role in caffeine-hyperresistance.</div>
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<DateCompleted><Year>2020</Year>
<Month>01</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>01</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1573-0972</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>35</Volume>
<Issue>12</Issue>
<PubDate><Year>2019</Year>
<Month>Nov</Month>
<Day>14</Day>
</PubDate>
</JournalIssue>
<Title>World journal of microbiology & biotechnology</Title>
<ISOAbbreviation>World J Microbiol Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.</ArticleTitle>
<Pagination><MedlinePgn>183</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s11274-019-2762-2</ELocationID>
<Abstract><AbstractText>Caffeine is a naturally occurring alkaloid, where its major consumption occurs with beverages such as coffee, soft drinks and tea. Despite a variety of reports on the effects of caffeine on diverse organisms including yeast, the complex molecular basis of caffeine resistance and response has yet to be understood. In this study, a caffeine-hyperresistant and genetically stable Saccharomyces cerevisiae mutant was obtained for the first time by evolutionary engineering, using batch selection in the presence of gradually increased caffeine stress levels and without any mutagenesis of the initial population prior to selection. The selected mutant could resist up to 50 mM caffeine, a level, to our knowledge, that has not been reported for S. cerevisiae so far. The mutant was also resistant to the cell wall-damaging agent lyticase, and it showed cross-resistance against various compounds such as rapamycin, antimycin, coniferyl aldehyde and cycloheximide. Comparative transcriptomic analysis results revealed that the genes involved in the energy conservation and production pathways, and pleiotropic drug resistance were overexpressed. Whole genome re-sequencing identified single nucleotide polymorphisms in only three genes of the caffeine-hyperresistant mutant; PDR1, PDR5 and RIM8, which may play a potential role in caffeine-hyperresistance.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sürmeli</LastName>
<ForeName>Yusuf</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Agricultural Biotechnology, Faculty of Agriculture, Namık Kemal University, 59030, Tekirdağ, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Holyavkin</LastName>
<ForeName>Can</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Topaloğlu</LastName>
<ForeName>Alican</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Arslan</LastName>
<ForeName>Mevlüt</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Genetics, Faculty of Veterinary Medicine, Van Yüzüncü Yıl University, 65080, Van, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kısakesen</LastName>
<ForeName>Halil İbrahim</ForeName>
<Initials>Hİ</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Çakar</LastName>
<ForeName>Zeynep Petek</ForeName>
<Initials>ZP</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-2278-3670</Identifier>
<AffiliationInfo><Affiliation>Department of Molecular Biology and Genetics, Faculty of Science & Letters, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey. cakarp@itu.edu.tr.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Dr. Orhan Öcalgiray Molecular Biology, Biotechnology and Genetics Research Center (İTÜ-MOBGAM), Istanbul Technical University, Maslak, 34469, Istanbul, Turkey. cakarp@itu.edu.tr.</Affiliation>
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<Language>eng</Language>
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<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>11</Month>
<Day>14</Day>
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<MedlineTA>World J Microbiol Biotechnol</MedlineTA>
<NlmUniqueID>9012472</NlmUniqueID>
<ISSNLinking>0959-3993</ISSNLinking>
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<NameOfSubstance UI="D018528">ATP-Binding Cassette Transporters</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018797">Cell Cycle Proteins</NameOfSubstance>
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<Chemical><RegistryNumber>06TPT01AD5</RegistryNumber>
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<Chemical><RegistryNumber>11118-72-2</RegistryNumber>
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<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
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<Keyword MajorTopicYN="N">Evolutionary engineering</Keyword>
<Keyword MajorTopicYN="N">Pleiotropic drug resistance (PDR)</Keyword>
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Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.

Evolutionary engineering and molecular characterization of a caffeine-resistant Saccharomyces cerevisiae strain.

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